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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been observed in several tumors, which ends up in chromosome instability, and therefore might contribute to tumorigenesis. This protein was located to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two further splice variants ended up described but comprehensive nucleotide sequence information and facts is just not however out there.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded through the antibody.

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This manual outlines further possible optimization of mobile and immunoassay parameters to be certain the very best final results are...

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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...

Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates for the G1-S period boundary and is degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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